Fig. 1.
Treatment of established tumor. a Vaccination protocol. B6 mice were given 1 × 105 viable B16-OVA melanoma cells s.c. in the right flank. Six days later, when the tumor size reaches 3–4 mm in diameter, young and old OVA-peptide(257–264) pulsed-DCs (PP-DCs), or PBS were injected once s.c. in the left flank. iDCs purified from recombinant mouse GM-CSF and IL-4 stimulated old and young bone marrow cells were pulsed with OVA-peptide at 10 μg/ml for 6 h. b Graph showing tumor growth over time. These data are representative of three experiments with similar results, and are reported as the mean tumor area ±SEM of five or six mice per group. *P < 0.001: young PP-DCs versus PBS control, old PP-DCs versus PBS control; ** P < 0.0005: young PP-DCs versus old PP-DCs. Copyright (c) The Gerontological Society of America. Reproduced by permission of the publisher [11]