Fig. 3.

In vivo lymph node DC migration in aging. a Groups of young mice (n = 3) received 50 μl of equal numbers (2 × 10⁶) of CMPTX-labeled young DC and CFSE-labeled old LPS-stimulated DCs into their footpad hind. Popliteal draining LNs were excised 24 h later and the frequency of DC/node was assessed by fluorescent microscopy. b Effect of increasing number of old DCs on tumor growth. Day-6 tumor-bearing mice were treated with 2 × 10⁶ young PP-DCs (1×), 2 × 10⁶ old PP-DCs (1×) or 4 × 10⁶ old PP-DCs (2×) or saline (DPBS), and tumor size measured 7 days later (n = 6 tumors per treatment group). Adapted from [12]