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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Neurochem. 2009 Aug 13;111(2):510–521. doi: 10.1111/j.1471-4159.2009.06335.x

Fig. 4.

Fig. 4

Restoration of hippocampal neuronal development inhibited by in vivo DHA-depletion by subsequent DHA supplementation in vitro. (a) Time line of the experimental design. E18 hippocampal neuronal cultures were obtained from pregnant mice fed adequate (Ade) or deficient (Def) diets for 16 days during pregnancy (E2–E18). The culture was grown in chemically defined medium in the presence or absence of 1 μM fatty acids for 10 days. (b–d). Total neurite length/neuron (b), number of synapsin-positive puncta/neuron (c), and number of synapsin-positive puncta/10 μm neurite (d) which were reduced by in vivo DHA-depletion but restored by subsequent DHA supplementation in vitro. Statistical analysis was performed by post hoc Tukey's HSD test at the significance level of p < 0.05. Different alphabetical letters indicate statistically significant differences.