Skip to main content
. Author manuscript; available in PMC: 2009 Nov 5.
Published in final edited form as: Anal Biochem. 2008 Aug 20;383(1):44–51. doi: 10.1016/j.ab.2008.08.009

Fig. 2.

Fig. 2

Rate of [3H]benzyl transfer to AGT in intact HL-60 and L1210/AGT1 cells. Cells (2 × 106 cells/100 μl) were incubated with 1 μCi of [3H]BG in the absence or presence of 20 μM MG132 or 20 μM CHX for the indicated periods of time, and radioactivity in a 70% methanol-insoluble fraction was determined. Radioactivity in the presence of excess unlabeled O6-BG was subtracted from the total radioactivity. The bottom panels show the plots used to determine the second-order kinetic constant (k).