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AGT assay using cell homogenates. (A) Effects of various ingredients on AGT activity were measured using HL-60 cell homogenates prepared in H2O(5 × 106 cells/ 100 μl). PMSF, phenylmethylsulfonylfluoride. (B) Kinetics of [3H]benzyl transfer using HL-60 cell homogenates (5 × 106 cells/100 μl) prepared in a buffer (pH 7.5) containing 1 mM DTT and plots to determine the second-order kinetic constant (k).
