. Author manuscript; available in PMC: 2009 Nov 5.

Published in final edited form as: Anal Biochem. 2008 Aug 20;383(1):44–51. doi: 10.1016/j.ab.2008.08.009

Table 1.

AGT levels in various human and rodent cell lines

Human AGT k = 1100 M^-1 s^-1 A₀ = 1.2 C (2 h incubation)		Murine AGT k = 380 M^-1 s^-1 A₀ = 2.2 C (2 h incubation)
Human leukemia cell lines		Murine suspension cell lines
U-937	<500	L1210	<500
TF-1	<500	P388	<500
K-562	<500	F-MEL	<500
NB4	8500	HGPRT^– F-MEL	<500
CCRF-CEM	14,000	FDC-P1	<500
Jurkat	15,000	B6SUtA	1400
Raji	17,000	Ba/F3	3200
HL-60	17,000	WEHI-3B D⁺	3400
Human carcinoma cell lines		Murine attached cell lines
U251	<500	EMT6	<500
HCT 116	1500	NIH/3T3	2600
A427	1700	B₁₆F₁₀	N.M.
A549	6600
Daoy	8800	Hamster attached cell line
HeLa S3	22,000	CHO	<500
MCF7	42,000
DU145	42,000	Rat attached cell line
LNCaP	45,000	NRK	2900

Note. Values are AGT molecules/cell. AGT assays using the standard conditions were conducted for 2 h. The saturation levels (A₀) were calculated by multiplication of the experimental values (C) and the constant determined by k. N.M., not measurable due to erroneous incorporation of [³H]BG.