Table 1. Bacterial strains and plasmids.
| Strains | Relevant genotype | Source/reference |
| MG1655a | F− λ− rph-1 | [47], [48] |
| LJ52b | MG1655 attB::pnrdA-lacZ | This work, [27] |
| SMG367 | MG1655/pDSW204 | Stèphanie Gon |
| SMG386 | MG1655ΔnrdAB/pIO5 | Stèphanie Gon |
| E101c | nrdA1 (ts) thyA6 deoB37 deoC1 thr-1 leuB6 fhuA21 lacY1 glnV44 λ− rfbC1 rpsL67 thi-1 | CGSC# 5146 |
| IO08 | E101/pIO5 | This work |
| HC123c | dnaN159 (ts) | CGSC# 6844 |
| MOR237 | MG1655 recA56 srl300::Tn10 | This work |
| Plasmids | ||
| pDSW204 | Promoter down mutation in −35 of pTrc99A | [49] |
| pIO5d | pDSW204-nrdAB (pSMG7) with mutation in nrdB (D367G)) | [50] |
a
Provided by Jon Beckwith.
b
In the experiment with HU (Figure 2, Table 2) the LJ52 strain was used. It is referred to as MG1655 for simplicity.
c
Obtained from The Coli Genetic Stock Center, Yale University.
d
The nrdAB gene on the plasmid pSMG7 [50] used in this study was found to contain a point mutation in nrdB that leads to an amino acid change at position 367 (D367G). The plasmid is referred to as pIO5 in the text.