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. 2009 Aug 3;114(18):3783–3792. doi: 10.1182/blood-2009-06-227843

Figure 4.

Figure 4

Long-term reconstitution of normal hematopoietic cells from leukemic marrow in new nonleukemic hosts. The hematopoietic regeneration of the normal hematopoietic cells from leukemic or control mice were examined in secondary nonleukemic recipients using the cBMT assay, in which equal numbers of test (CD45.1+GFP−) and competitor cells (CD45.1+/.2+) were cotransplanted into lethally irradiated congenic recipients (CD45.2+). The overall reconstitution levels of normal HSCs from the primary recipients were monitored within 6 months after transplantation (A). Multilineage differentiation of the engrafted cells was analyzed 6 months after transplantation (B). GM, T, and B indicate lineages for myeloid (Mac-1+), T (CD3+), and B (B220+) cells, respectively. Six months after cBMT, the overall representation of CD45.1+GFP− cells, multilineage analysis, and different hematopoietic cell subsets in BM were also quantified (C-E). *P < .05; **P < .01 (n = 5-7/group, t test). Data are from 1 of 3 experiments with similar results.