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. Author manuscript; available in PMC: 2009 Nov 5.
Published in final edited form as: Glia. 2007 Jan 15;55(2):119–129. doi: 10.1002/glia.20439

Fig. 7.

Fig. 7

NHERF1 interacts directly with the C-terminal ETKM motif of GLAST. (A) Sequence alignment of the mammalian GLAST C-terminal tails (generated using the Vector NTI program). Identical amino acids are depicted in grey shading and the PDZ binding consensus is boxed. (B) In vitro binding assay. GST fusion proteins containing the GLAST C-terminus (GST–GLASTct) and the GLAST C-terminus deletion mutant (GST–GLAST Δ-PDZ), as well as GST alone were separated on SDS-PAGE, transferred to nitrocellulose membrane and probed with [35S]-methionine-labeled NHERF1. Binding was detected by autoradiography.