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. Author manuscript; available in PMC: 2009 Nov 5.
Published in final edited form as: Channels (Austin). 2009 Jan 7;3(1):16–24. doi: 10.4161/chan.3.1.7387

Figure 2.

Figure 2

Co-expression of KCNE2, but not KCNE3, alters function of pseudo phosphorylated KCNQ1 channels. Whole-cell currents were measured with perforated patch-clamp configuration from CHO cells transfected with S27D_KCNQ1 and Yotiao plus either KCNE2 (A) or KCNE3 (B). The S27D mutation was used to mimic the PKA phosphorylation of the IKs channels at Ser-27 in KCNQ1 in the absence of cAMP16 (see text). Membrane currents were elicited by 2-s test pulses from −120 mV to 100 mV (20-mV increments) following 2-s pulses to −40 mV. Pulse frequency was 0.067 Hz. The membranes were held at −65 mV for KCNE2 and −80 mV for KCNE3. I–V curves (wild type KCNQ1 (WT): open squares, S27D KCNQ1 (SD): blue squares, S27A KCNQ1 (SA): red squares) were plotted with amplitudes at the first test pulses, which are pointed by arrows in the representative traces. The numbers of experiments were as follows: KCNE2 WT, n = 15, SD, n = 10, and SA, n = 7; KCNE3 WT, n = 6, and SD, n = 7. *p < 0.05 vs. WT KCNQ1, ANOVA for KCNE2 and Student’s t-test for KCNE3.