Figure 4.

The cell surface phenotype of WT and ΔE3 cells. (A): Immunofluorescence analysis of Pro-SPC (red) and CCSP (green) in lung section from WT (left column) and CCSP-Cre, Catnnb^{floxE3/floxE3} (ΔE3) animals (right column). The top row shows Pro-SPC staining, and the bottom row represents merged images of Pro-SPC and CCSP staining (magnification, ×200). Insets represent higher-magnification images of the outlined areas. (B): Sca-1 and CD34 expression on the surface of cells isolated from WT (top row) and ΔE3 animals (bottom row). In left-to-right order, the columns represent unstained, Sca-1-stained, CD34-stained, and Sca-1- and CD34 dual-stained samples, respectively. Dead and CD45^pos CD31^pos cells were excluded from this analysis. (C): Quantification of data in (B), comparing the percentages of cells in each population of Sca-1/CD34 dual-stained samples from WT (gray bars) and ΔE3 (white bars) mice. Abbreviations: CCSP, Clara cell secretory protein; PE, phycoerythrin; Pro-SPC, pro-surfactant protein C; WT, wild-type.