Figure 4.

(a) Original chromatograms and (b) logarithmic peak profiles for 100 µl injections of 10 µM racemic warfarin applied to a 2.5 mm × 2.1 mm I.D. HSA column or control column containing 7 µm silica particles. The flow rates used in these experiments (from left-to-right) were 4.0, 3.5, 3.0, 2.5, and 2.0 ml/min. The mobile phase was pH 7.0, 0.10 M potassium phosphate buffer and the temperature was 25°C.