Table 1.

Predicted plate height terms for racemic warfarin applied to a 2.5 mm × 2.1 mm I.D. HSA column at 25°C and various flow rates^a

Flow rate	Hsm	Hk	Hm	Hi	Hec	Htot	Hk/Htot	n
(ml/min)	(cm)	(cm)	(cm)	(cm)	(cm)	(cm)
0.1	0.0006	0.0052	0.0035	0.0229	0.0004	0.0326	0.1605	7.6606
0.5	0.0031	0.0262	0.0035	0.0205	0.0004	0.0537	0.4878	4.6574
1	0.0062	0.0524	0.0035	0.0189	0.0005	0.0814	0.6431	3.0700
1.5	0.0092	0.0785	0.0035	0.0172	0.0006	0.1091	0.7201	2.2919
2	0.0123	0.1047	0.0035	0.0183	0.0007	0.1396	0.7503	1.7910
2.5	0.0154	0.1309	0.0035	0.0125	0.0006	0.1629	0.8038	1.5350
3	0.0185	0.1571	0.0035	0.0133	0.0007	0.1931	0.8135	1.2946
3.5	0.0215	0.1833	0.0035	0.0126	0.0007	0.2217	0.8266	1.1275
4	0.0246	0.2095	0.0035	0.0145	0.0009	0.2530	0.8279	0.9881

^aThe equations and methods used to determine the individual plate terms in this table are summarized in the text. The number of theoretical plates (n) was calculated by using the relationship n = L/H_tot, where L is a column length of 2.5 mm in this particular example. An average value for racemic warfarin of 0.35 s⁻¹ was used for k_d and a value of 3.0 × 10⁵ M⁻¹ was used for K_a in this particular calculation.