Table 2.
Elution time of peak maximum (tp) and slopes of peak decay curves for racemic warfarin on the HSA column and control columns at 25°C and various flow ratesa
| Flow rate (ml/min) |
1 µM Warfarin on HSA column |
10 µM Warfarin on HSA column |
10 µM Warfarin on control column |
|||
|---|---|---|---|---|---|---|
| tp (s) | Slope (s−1) | tp (s) | Slope (s−1) | tp (s) | Slope (s−1) | |
| 0.1 | 231.3 | −0.015 (± 0.001) | 139.6 | −0.020 (± 0.001) | 82.4 | −0.051 (± 0.001) |
| 0.5 | 48.6 | −0.061 (± 0.002) | 29.2 | −0.086 (± 0.005) | 17.1 | −0.182 (± 0.002) |
| 1.0 | 25.7 | −0.094 (± 0.006) | 16.3 | −0.147 (± 0.012) | 10.0 | −0.443 (± 0.012) |
| 1.5 | 17.8 | −0.150 (± 0.007) | 11.9 | −0.196 (± 0.002) | 7.5 | −0.551 (± 0.009) |
| 2.0 | 14.2 | −0.162 (± 0.007) | 9.4 | −0.229 (± 0.009) | 6.2 | −0.666 (± 0.007) |
| 2.5 | 12.0 | −0.190 (± 0.006) | 8.2 | −0.250 (± 0.006) | 5.1 | −0.855 (± 0.006) |
| 3.0 | 9.9 | −0.212 (± 0.014) | 7.3 | −0.344 (± 0.003) | 4.8 | −0.915 (± 0.013) |
| 3.5 | 9.1 | −0.236 (± 0.022) | 6.7 | −0.347 (± 0.007) | 4.5 | −0.981 (± 0.015) |
| 4.0 | 8.0 | −0.248 (± 0.019) | 6.0 | −0.344 (± 0.007) | 4.2 | −1.226 (± 0.013) |
a
The values in parentheses represent a range of ± 1 S.D. All experiments in this table were performed using a 100 µl sample volume.