. Author manuscript; available in PMC: 2009 Nov 5.

Published in final edited form as: J Sep Sci. 2009 May;32(10):1632–1641. doi: 10.1002/jssc.200900074

Table 3.

Summary of dissociation rate constants determined by the peak decay method for warfarin from HSA

	Dissociation rate constant, k_d (s⁻¹)^a
Temperature	R-Warfarin	S-Warfarin	Racemic warfarin
4°C	0.39 (± 0.01)	0.44 (± 0.01)	---
37°C	0.56 (± 0.01)	0.66 (± 0.01)	---
25°C	---	---	0.35 (± 0.02)^b

The values for the individual enantiomers of warfarin were measured in pH 7.4, 0.067 M potassium phosphate buffer, while the value for the racemic mixture was measured in pH 7.0, 0.10 M potassium phosphate buffer. The values in parentheses represent a range of ± 1 S.D.

Individual estimates for k_d ranged from 0.34 s⁻¹ to 0.37 s⁻¹ for the various columns and analysis conditions used in this study.