Figure 4. Effects of long-term hyperglucagonaemia on glomerular mesangial expansion and extracellular matrix deposition in glucagon-infused mice.

Kidney sections from control (A), glucagon-infused mice (B) and mice treated concurrently with glucagon and [Des-His1-Glu9]glucagon (C) were stained with PAS. Mesangial expansion and extracellular matrix deposition are indicated by the arrows. The extent of glomerular injury was expressed as a percentage of glomerular volume (D). Values are means ± S.E.M. (n = 6). **P < 0.01 compared with control mice; ++P < 0.01 compared with glucagon-infused mice. Ant, [Des-His1-Glu9]glucagon.