Figure 5. Effects of long-term glucagon infusion on total and phospho-ERK 1/2 in mouse liver (A) and kidney (B), as measured using Western blotting.

p-ERK1/2, phospho-ERK1/2 (Tyr204); t-ERK1/2, total ERK1/2. Quantification of phospho-ERK1/2 is shown below the immunoblots. Values are means ± S.E.M. (n = 6). **P < 0.01 compared with control; ++P < 0.01 compared with glucagon-infused mice. Ant, [Des-His1-Glu9]glucagon.