Fig. 1.

Macrophages activated with IFN-γ plus LPS exhibit increased levels of stable MTs. RAW264.7 cells were stimulated with IFN-γ/LPS (100 units/ml and 1 μg/ml, respectively, for 12 h) or left untreated. A, cells were fixed and immunostained for acetylated tubulin (red) and α-tubulin (green). Scale bar, 10 μm. B, Western blot analysis of total cell lysates. A representative Western blot shows the acetylated tubulin and total α-tubulin levels in resting and IFN-γ/LPS-treated RAW264.7 cells. C, densitometric analysis revealed a substantial and significant increase in acetylated tubulin levels in IFN-γ/LPS-treated macrophages compared with control macrophages. Data is expressed as mean ± S. E. (n = 3). * indicates p < 0.05.