Table 1. Primers used to amplify the PAX6 gene.
| Exon | Amplified length (bp) | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) |
|---|---|---|---|
| 1 |
645 |
GCATGTTGCGGAGTGATTAG |
CTCCTGCGTGGAAACTTCTC |
| 2 |
645 |
GCATGTTGCGGAGTGATTAG |
CTCCTGCGTGGAAACTTCTC |
| 3 |
676 |
AGAGAGCCCATGGACGTATG |
GTCGCGAGTCCCTGTGTC |
| 4 |
676 |
AGAGAGCCCATGGACGTATG |
GTCGCGAGTCCCTGTGTC |
| 5 |
373 |
TGAGGATGCATTGTGGTTGT |
GAAATGAAGAGAGGGCGTTG |
| 6 |
388 |
CGTAAGCTTGTCATTGTTTAATGC |
AGAGAGGGTGGGAGGAGGTA |
| 7 |
333 |
GGTTGTGGGTGAGCTGAGAT |
AAGCCCTGAGAGGAAATGGT |
| 8 |
355 |
GGCTGTCGGGATATAATGCT |
CAAAGGGCCCTGGCTAAAT |
| 9 |
385 |
AGGTGGGAACCAGTTTGATG |
TGGGACAGGTTAGCACTGTGT |
| 10 |
537 |
AGCAGTGGAGGTGCCAAG |
TCTCAAGGGTGCAGACACAG |
| 11 |
537 |
AGCAGTGGAGGTGCCAAG |
TCTCAAGGGTGCAGACACAG |
| 12 |
345 |
CAGACTTGTTGGCAGAGTTCC |
TAAACACGCCCTCCCATAAG |
| 13 | 373 | TTTCTGAAGGTGCTACTTTTATTTG | CGGCTCTAACAGCCATTTTT |