Figure 2.

Identification of a KP-element insertion mutation in the ras gene. (A) A cytological map showing the deletions uncovering the mutation to the genomic region spanning the breakpoints at 85D19 and 85D24. Below the map is an overlay of the genomic annotation for this region containing 14 genes http://flybase.org. (B) The genomic organization of the ras gene showing the position of the KP element insertion. Black boxes indicate coding sequences, white boxes are the UTR sequences, and the arrows indicate the direction of gene transcription. (C) Normalized real-time PCR data where control level of ras gene expression is set to 1. ras expression level is markedly reduced in the ras^KP/ras^KP mutant, especially in the adult flies. (D) Constitutive expression of wild-type Ras by the actin promoter fully rescues the ras^KP mutant eye phenotype (left panel), as well as body size (right panel).