Table 1.
Primers and annealing temperatures used for RT-PCR
| Gene | Sequence (5'-3') | Product size (bp) | Annealing temperature (°C) |
| β-actin-F | TTCCTTCTTGGGTATGGAAT | 200 | 55 |
| β-actin-R | GAGCAATGATCTTGATCTTC | ||
| AFP-F | CACTGCTGCAACTCTTCGTA | 300 | 52 |
| AFP-R | CTTTGGACCCTCTTCTGTGA | ||
| HNF3β-F | GACCTCTTCCCTTTCTACCG | 551 | 51 |
| HNF3β-R | TTGAAGGCGTAATGGTGC | ||
| TTR-F | TGCCTCGCTGGACTGGTAT | 334 | 52 |
| TTR-R | CAGAGTCGTTGGCTGTGAA | ||
| DPPIV-F | GATTCCATACCCAAAGGC | 587 | 55 |
| DPPIV-R | GGTCACAACTAAGGCACT | ||
| ALB-F | TCTTCGTCTCCGGCTCTG | 475 | 55 |
| ALB-R | CTGGCAACTTCATGCAAA | ||
| OCT4-F | GGCGTTCTCTTTGGAAAGGTGTTC | 313 | 57 |
| OCT4-R | CTCGAACCACATCCTTCTCT | ||
| AAT-F | AGAACCATTATCAGGCAGAA | 675 | 55 |
| AAT-R | AATAAGGAACGGCTAGTAAGA | ||
| DLK-F | GGGGTGACTTCCGTTGC | 510 | 52 |
| DLK-R | GCTCCTCGCCGCTGTTAT | ||
| HNF4-F | CTTCCAAGAGCTGCAGATTG | 517 | 55 |
| HNF4-R | CTTGTAGGATTCAGATCCCG | ||
| G6P-F | TCAATCTCCTCTGGGTGGC | 602 | 52 |
| G6P-R | GGCAAAGGGTGTAGTGTCAAG | ||
| TAT-F | CTTCAGTGCTGGATGTTCGC | 619 | 55 |
| TAT-R | CAGGGATTGGACGGGTTGTT | ||
| TDO-F | TAAACAGAGCCAGCAAAG | 868 | 56 |
| TDO-R | ATGAGCGTGTCAATGTCC | ||
| BG-F | CGTGAAGGATACGGGAGT | 581 | 55 |
| BG-R | CAGAGTTATTGACGAGGC | ||
| GGT-F | TGTCCCTGGTGAAATCCG | 577 | 55 |
| GGT-R | GGCATAGGCAAACCGAAA |
AFP: α-fetoprotein; HNF: Hepatocyte nuclear factor; TTR: Transthyretin; DPPIV: Cytokeratin 18; ALB: Albumin; OCT4: Octamer-4; AAT: α-1-antitrypsin; Dlk: Δ-like (Pref-1, preadipocyte factor-1); G6p: Glucose-6-phosphatase; TAT: Tyrosine aminotransferase; TDO: Tryptophan 2,3-dioxygenase; BG: Biliary glycoprotein; GGT: γ-glutamyl transpeptidase.