Skip to main content
. 2000 Aug 22;97(18):10120–10125. doi: 10.1073/pnas.170192197

Figure 4.

Figure 4

Association of StIP1 with Stat3 and JAKs. (A) Extracts were prepared from M1 cells either before or after stimulation with IL-6 (200 units/ml). They were immunoprecipitated either with a StIP1 or Stat3 (C-20, Santa Cruz Biotechnology) antibody and subsequently immunoblotted with either a Stat3 (◂; C-20, Santa Cruz Biotechnology), StIP1 (▹), or antiphosphotyrosine (4G10; Upstate Biotechnology) antibodies as indicated. (B) M1 extracts were immunoprecipitated with Jak1 (lanes 1 and 2; Upstate Biotechnology), Jak2 (lanes 3 and 4; Upstate Biotechnology), or Tyk2 (lanes 5 and 6) antibodies and sequentially immunoblotted with StIP1 or the appropriate JAK antibodies as indicated. (C) Extracts from unstimulated (lanes 1–6) and IL-6-stimulated (lanes 7–12) M1 cells were incubated with GST-StIP1 fusion proteins (lanes 3–6 and 9–12) or GST alone (lanes 2 and 8) and bound proteins detected with a Jak1-specific antibody (←; Signal Transduction Laboratories, Lexington, KY).