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. 2009 Nov 18;4(11):e7886. doi: 10.1371/journal.pone.0007886

Figure 6. Rac activation is dependent on the stress-magnitude.

Figure 6

Bar graphs represent the normalized emission ratios of the YPet/CFP Rac biosensor in human airway smooth muscle (HASM) cells and SYF−/− MEFs at the time point of 0.3 s upon stress application. There were no significant differences in Rac activation between 1.8 and 3.5 Pa stress, or between 3.5 and 8.8 Pa stress (p>0.05), but there were significant differences between 8.8 and 17.5 Pa stress (p<0.01), for either HASM or SYF−/− MEF cells. These data suggest that stress threshold for Rac activation is between 8.8 and 17.5 Pa. At 1.8 Pa, n = 6 or 7; at 3.5 Pa, n = 5 or 7; at 8.8 Pa, n = 5 or 8; at 17.5 Pa, n = 6; for HASM or SYF−/− MEFs, respectively. Mean +/− s.e.