Fig. 4.

Analysis of binding by ITC. Binding constants were determined from sigmoidal fits to two independent measurements. (A) Representative ITC measurements for ABP, Lac.A1, and Stn.A2. No significant change in heat upon addition of the respective ligand could be detected in the case of the designs. (B) Representative ITC measurements for GBP, Lac.G1, and PMPA.G12. No change in heat upon addition of the respective ligand could be detected in the case of the designs. (C) Representative ITC measurement for wild-type RBP. Saturation is reached for wild-type RBP already at a molar ratio of 0.5, which indicates that residual ribose remains in the purified protein sample. No ITC measurement could be performed with TNT.R1 because the ligand TNT is insoluble in aqueous buffer.