Skip to main content
. 2009 Oct 21;106(44):18604–18609. doi: 10.1073/pnas.0900635106

Fig. 4.

Fig. 4.

HA synthetase, Has2, activated by Tbx2 in cardiac chamber myocytes. (A) Real-time PCR analysis of dissected embryonic hearts at E9.5 or E9.75. Has2 transcripts were tripled in the ventricles of activated mTbx2 embryos. (B) Whole-mount in situ hybridization analysis for Has2 expression was detected in the AVC (black arrowheads) of control littermates whereas expanded to ventricles (red arrowheads) of activated Tbx2 embryos. The middle and right columns show sections after WISH. Has2 expression was activated in ventricular myocardial cells of activated Tbx2 embryos (right column). Nuclear were stained by nuclear fast red.