Fig. 3.

Forced overexpression of miR-15a, miR-16, and miR-150 in T-ALL and primary ACC cells. (A) Q-PCR analyses of MYB mRNA expression in MOLT-4 cells with duplication/activation of MYB and in MYB-NFIB–expressing primary ACC2 and -6 cells after transfection with a pool of premiR-15a, premiR-16, and premiR-150 oligos. The data represent the average of at least 3 independent experiments. T, transfected; UT, untransfected. (B) Transfection of primary ACC6 cells with a Cy3-labeled premiR negative control shows a transfection efficiency of more than 95%. BF, bright field.