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. 2009 Oct 19;106(44):18598–18603. doi: 10.1073/pnas.0904894106

Fig. 3.

Fig. 3.

Wnt regulates cellular distribution of Gpr177. (A–D) Three-dimensional images of immunostained cells reveal differential localizations of endogenous Gpr177 in NEP (A), Neurosphere cells (B), MSC (C), and C57MG (D). Cut view (E–I) and 3-D imaging (J–O) analyses show that Gpr177 co-localizes with a Golgi marker GM130 using superimposed imaging (H and L) or pseudocoloring of the co-localization signal in white (I and M–O). NEP cells were immunostained with Gpr177 (E and J) and GM130 (F and K), and counterstained with DAPI (G–O). Three serial sections along the front-view (Co Level 1, 2, and 3) reveal the co-localization signal on the surface of Golgi (M–O). (P–T) High levels of Gpr177 lead to its accumulations in Golgi. Three-dimensional imaging of the endogenous Gpr177 in the vesicles of C3H10T1/2 cells (P). Expression of a myc-tag full length Gpr177 (MT-Gpr177) shows alteration in subcellular distribution of Gpr177 (Q), co-localizing with GM130 (R–T). Co-immunostaining of a MT-Gpr177 mutant lacking the carboxyl terminal region (U) and Calnexin (V) reveals their co-localization (W).