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. 2009 Oct 19;106(44):18503–18508. doi: 10.1073/pnas.0910392106

Fig. 3.

Fig. 3.

Mutant sul20C tags alter Lon activity. (A) SDS/PAGE assays of Lon6 (0.15 μM) degradation of titin-I27CM (5 μM) bearing mutant sul20C tags. (B) Steady-state rates of Lon6 (0.3 μM) degradation of different concentrations of 35S-titin-I27CM bearing wild-type, YàA (penultimate residue), or HàA (C-terminal residue) sul20C tags were fit to the Hill equation. (C) ATP hydrolysis by Lon6 (0.15 μM) as a function of [titin-I27CM-sul20C] bearing the penultimate YàA mutation or C-terminal HàA sul20C mutations. Lines are fits to the equation rate = basal + (Vmax-basal)·[S]/(K0.5 + [S]). (D) Rates of Lon ATP hydrolysis at saturating concentrations of titin-I27CM with different degradation tags. Values are averages (n = 3) ± 1 SD. (E) The energetic efficiency of Lon degradation varies dramatically for titin-I27CM variants with different degrons. Error bars are 1 SD.