Table 1.

Levels of let-7-Fam miRNAs are specifically reduced in daf-12 mutants or in crowded environments

miRNA	Fold change wild type versus mutant			Sparse vs. crowded
	daf-12(rh61)	daf-12(rh61rh411)	din-1; daf-12(rh61)	Wild type
let-7 family
mir-48	−16.0 ± 4.2	−3.2 ± 0.5	−3.5 ± 0.8	−3.2 ± 1.3
mir-241	−17.8 ± 4.7	−4.3 ± 0.7	−4.5 ± 1.1	−9.4 ± 3.8
mir-84	−2.7 ± 0.7†	−2.8 ± 0.4†	−1.7 ± 0.6	−2.6 ± 0.7
let-7	−12.2 ± 5.5	−12.3 ± 2.5	−6.0 ± 1.6	off*
mir-793	+0.3 ± 0.2	−0.1 ± 0.3	+8.5 ± 4.3†	+0.2 ± 0.3
mir-794	Off*	Off*	Off*	Off*
mir-795	−31.0 ± 17.8	−5.0 ± 1.2†	−3.8 ± 1.0†	−11.7 ± 5.2
lin-4 family
lin-4	+0.7 ± 0.2	+0.1 ± 0.1	−0.03 ± 0.3	+0.3 ± 0.2
mir-237	−1.2 ± 0.4	−0.1 ± 0.2	−0.75 ± 0.2	−0.7 ± 0.4
Other miRNAs that were significantly reduced‡
mir-230	−3.4 ± 1.5	−1.3 ± 0.2	−1.1 ± 0.2	−0.1 ± 0.2
mir-85	−18.7 ± 5.9	−2.1 ± 0.6	−11.0 ± 2.7	−1.3 ± 0.7
mir-788	−1.7 ± 0.5	−1.7 ± 0.3	−0.3 ± 0.2	2.4 ± 0.7

Values in boldface indicate miRNA significantly changed, P < 0.01. Negative values indicate miRNA decreased in daf-12 relative to wild type, or in crowded relative to sparse conditions. Values close to 0 indicate miRNA was unchanged. Data are displayed as (mean fold-change) ± (mean estimated noise). For details see Materials and Methods in SI Text.

*Levels were beneath the limit for detection.

^†Levels changed in only one biological replicate (see SI Text).

^‡No miRNAs were significantly increased in daf-12 mutants relative to wild type. However, crowded wild-type larvae showed a significant increase (2–24 times) of mir-242, mir-243, mir-34, mir-71 and mir-792.