Table 2.
Effects on daf-12-dependent activity by reduction of let-7-Fam miRNA levels
| nM DA ligand | daf-9 | mir-241; daf-9 mir-84 | mir-48 mir-241; daf-9 mir-84 |
|---|---|---|---|
| Mig | |||
| 25 | 83 ± 15 (63) | 81 ± 15 (24) | ND |
| 50 | 35 ± 17 (88) | 82 ± 9 (61) | 78 ± 16 (52) |
| 100 | 7 ± 4 (150) | 34 ± 21 (127) | ND |
| dauer | |||
| 0 | 100 ± 0 (46) | 100 ± 0 (33) | ND |
| 25 | 25 ± 13 (138) | 39 ± 4 (95) | ND |
| 50 | 5 ± 2 (108) | 3 ± 1 (79) | 19 ± 8 (95) |
| 100 | 0 ± 0 (150) | 0 ± 0 (127) | ND |
Data are presented as percentage of animals exhibiting the relevant phenotype, plus or minus the standard error of the mean for three independent trials. Total number of animals scored is in parentheses. ND, not determined.