Skip to main content
. 2009 Oct 19;106(44):18769–18774. doi: 10.1073/pnas.0910561106

Fig. 3.

Fig. 3.

Histological analysis of ischemic adductor muscles from diabetic mice after therapy. (A–D) Hematoxylin-eosin staining of tissue sections on day 7 after femoral artery ligation and treatment with AdLacZ (A) or AdCA5 (B) (original magnification, 40×). Black arrow indicates area infiltrated with inflammatory cells and red arrow indicates area with preservation of normal muscle tissue histology. Boxed areas are shown at higher power (C and D; original magnification, 200×). (E–J) Immunohistochemical staining of infiltrated areas for macrophages (E and F), lymphocytes (G and H), and neutrophils (I and J) using F4/80, CD3 and myeloperoxidase antibodies, respectively (original magnification, 200×). (K) quantification of infiltrated and preserved areas as percentage of total tissue area with mean ± SEM (n = 4 mice each) shown. **, P < 0.01 (Mann–Whitney test).