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. 2009 Oct 19;106(44):18769–18774. doi: 10.1073/pnas.0910561106

Fig. 6.

Fig. 6.

Analysis of eNOS activity in the calf muscles of diabetic mice. Immunoblot assays (A) were performed to quantify the levels of total eNOS protein (B) and eNOS phosphorylated at Ser-1177 (p-eNOS, C) in ischemic (ISC) and nonischemic (NIS) muscles on day 7 after femoral artery ligation. The expression of eNOS or p-eNOS was normalized to the β-actin level in the same lysate and the ISC:NIS ratio was determined. *, P < 0.05 vs. contralateral NIS limbs; #, P < 0.05 vs. AdLacZ-treated ISC limbs (Mann-Whitney test). Mean ± SEM is shown (n = 5–6).