Figure 3.

Interferon-β induces innate DNA recognition receptors in GEnC. A: GEnC were cultured in the presence or absence of various concentrations of IFN-β as indicated. Total mRNA was harvested after 6 (black bars) or 12 hours (gray bars) of stimulation and real-time reverse transcription-PCR was performed for ZBP1/DAI and the type I interferon receptor (IFNAR) as indicated. Data are expressed as ratio of the specific mRNA per respective 18S rRNA expression. *P < 0.05 vs. medium. B: In a similar experiment GEnC extracts were harvested after 24 hours for Western blotting with specific antibodies for ZBP1/DAI and phosphorylated (P-) IRF3. Blots shown are representative for each of three comparable experiments.