Figure 5.

B-DNA activates GEnC to express adhesion molecules and enhances leukocyte adhesion in vivo. A: GEnC surface expression of ICAM-1 and VCAM-1 was determined by flow cytometry after stimulation with various concentrations B-DNA, cationic lipids, and LPS as indicated. Data represent means ± SEM of four experiments. *P < 0.05. B: In vivo microscopy was performed on cremaster muscle postcapillary venules as described in methods. Four mice in each group were treated with intrascrotal injections of vehicle, cationic lipid (CL), B-DNA/CL, B-DNA/CL+ isotype IgG control, and B-DNA/CL+anti ICAM-1 as indicated in the legend. Leukocyte rolling, firm adhesion, and transendothelial migration were determined 6 hours after injection and are shown as means ± SEM **P < 0.05 vs. PBS.