Figure 2.

Effect of hypoxia-ischemia on LC3 distribution. A and B: Immunohistochemistry shows a strong increase in LC3 expression from 6 hours after the insult, and high-magnification views show numerous LC3-positive dots (vesicles corresponding to autophagosomes) at 24 hours in the cortex (A) mainly in the border of the lesion (left side of the dotted line) but also in some cells inside the lesion (right side of the dotted line and lower side in high magnification) and in the hippocampus essentially in CA3 (B). High magnifications of two or more representative neurons are shown for the cortex and CA3 illustrating the increase in the granular labeling of LC3 from 6 hours after hypoxia-ischemia. C: No change is detectable in CA1. D and E: Increase in punctate LC3 occurs in neurons as shown by confocal microscopy of double immunolabeling at 24 hours for NeuN (red) and LC3 (green) in cortex (D) and CA3 (hippocampus, E). Scale bars: 10 μm.