Table 1.

Fold Change in Steady-State Proinflammatory Transcript Expression in 3×Tg-AD Mice Injected with rAAV1-IFNγ versus rAAV1-eGFP at 10 Months Post-Transduction

Target Gene	Fold-change rAAV1-IFNγ- versus rAAV1-eGFP-infused 3×Tg-AD mice	SEM	P values for significance (Student t-test)
CCL2 (MCP-1)	2.1399	0.3565	<0.01
CCL3 (MIP-1α)	1.8079	0.1982	<0.01
CCL6 (MRP-1)	1.8787	0.0937	<0.001
TNF-α	2.9663	0.6033	<0.01
TNFrsf1a	1.4606	0.0754	<0.001
TNFrsf1b	1.4793	0.1261	<0.01
C3	−1.5346	0.1436	<0.05
Jun	1.8071	0.0673	<0.001
NFκb2	2.2097	0.1907	<0.001

Transcriptional analysis of 3×Tg-AD mice injected with rAAV1-eGFP or rAAV1-IFNγ for additional proinflammatory markers of interest. Total RNA was purified from microdissected hippocampus from 12-month-old 3×Tg-AD mice that had been previously stereotactically infused with rAAV1-IFNγ or rAAV1-eGFP. cDNA pools were generated and subjected to Applied Biosystems Microfluidic Card analysis of additional inflammation-related transcriptional targets. Fold changes in steady-state transcript levels in rAAV1-IFNγ- versus rAAV1-eGFP-injected animals were calculated with SEM and subsequently tested for significance by Student’s t-test.