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. 2009 Nov;175(5):2111–2120. doi: 10.2353/ajpath.2009.090257

Figure 5.

Figure 5

Unilateral transient CCA occlusion did not induce preconditioning. A: Representative Western blots showing p-Akt (60 kDa), p-GSK-3β (46 kDa), and p-ERK1/2 (42/44 kDa) in sham-operated (Sham) and uni-CCAo (model M2) neocortical cytosolic extracts at 48 hours postlesion compared with total (t) protein. No significant difference between M2 and sham-operated animals in p-Akt and p-GSK-3β was detected. An increase (∼50%) in p-ERK2 was only detected. B: Effect of uni-tCCAo 24 hours before ischemia on the lesion score (lesion was graded from 0 to 3, where 0 indicated no observable lesion and 1, 2, and 3 indicated small, medium and large infarct, respectively, see Ref. 11). Representative Cresyl-violet stained sections (upper panels) showing a similar pale zone in animals subjected to previous uni-tCCAo (model M5) as compared with ischemic animals (model M1). Lesion score also was similar in both groups (lower panel, n = 6, each).