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. 2009 Nov;175(5):2121–2132. doi: 10.2353/ajpath.2009.090418

Figure 2.

Figure 2

Protective effect of microglia activated with CpG against oAβ1-42 neurotoxicity. A: Representative deconvolution fluorescent images of control neuron-microglia co-cultures (1:2 of neuron: microglia). B: Neuronal cultures treated with 5 μmol/L oAβ1-42. C: Neuron-microglia co-cultures (1:2 neuron to microglia) treated with oAβ1-42. D: Neuron-microglia co-cultures stimulated with 1 nmol/L CpG and oAβ1-42 (E) or with 10 nmol/L CpG and oAβ1-42 (F) or with 100 nmol/L CpG and oAβ1-42. After 3 hours of treatment with CpG, cultures were treated with oAβ1-42 for 24 hours. Neurons were stained with an anti-MAP-2 antibody (green). Aβ was stained with 4G8 (red) and microglia were stained with a phycoerythrin-conjugated anti-CD11b antibody (blue). Scale bar, 50 μm. G: Neuronal survival rate was quantified as the percentage of intact neurons following treatment relative to control wells. The viability of untreated neurons (control) was normalized to 100%. ∗P < 0.05 as compared with the survival rate of neuron-microglia co-cultures treated with oAβ1-42. Each column indicates the mean ± SEM (n = 7).