Skip to main content
. 2009 Nov;175(5):2121–2132. doi: 10.2353/ajpath.2009.090418

Figure 6.

Figure 6

Effect of ICV injection of CpG on associative learning in the cued and contextual conditioning tests and Aβ clearance in Tg2576 mice. The retention session was performed 24 hours after the training. Context-dependent (A) and tone-dependent (B) freezing times were measured at the age of 10 months. Each column indicates the mean ± SEM (wild-type (WT) mice, n = 14; vehicle-treated (PBS) Tg2576 mice, n = 9; CpG (10 nmol/L)-treated Tg2576, n = 8; CpG (100 nmol/L)-treated Tg2576, n = 8). ∗P < 0.05 compared with wild type. **P < 0.05 as compared with vehicle-treated Tg2576 mice. C: Aβ deposits in the cortex. Aβ was stained with 4G8 (green), microglia stained with anti-CD11b antibody (red), and cell nucleus stained with Hoechst 33342 (blue). White arrows indicate Aβ deposits surrounding by microglia. Scale bar, 200 μm. D: Aβ deposits in the hippocampus. Scale bar, 200 μm. E: Quantification of Aβ deposits in C and D. The ratio between the Aβ deposits area and the total area of the analyzed region was multiplied by 100. ∗P < 0.05 and ∗∗P < 0.01 as compared with vehicle-treated Tg2576 mice. Each column indicates the mean ± SEM (n = 3). F: Western blot analysis of oAβ extracted from the hemi-forebrains of Tg 2576 mice. A 12-mer oAβ was detected in vehicle-treated Tg2576 mice and decreased in 100 nmol/L CpG-injected Tg2576 mice. G: Semiquantification of 12-mer oAβ by densitometric analysis. The amount of 12-mer oAβ1-42 in vehicle-treated Tg2576 mice was normalized to 100%. ∗P < 0.05 as compared with vehicle-treated Tg2576 mice. Each column indicates the mean ± SEM (n = 4).