Figure 2.

The HIF-1α inhibitors, echinomycin and emetine, interfere with induction of Cr-1 protein expression by hypoxia in mES cells. A: mES cells were incubated in a hypoxic chamber in the presence or absence of various concentrations of echinomycin (5 or 2.5 nmol/L) or emetine (0.25 or 0.5 μmol/L) for 24 hours and analyzed by Western blot using anti-Cr-1 or anti-β-actin antibodies. B: Fold difference in Cr-1 protein expression by desitometric analysis of Cr-1 in mES cell lysates normalized to β-actin content. Densitometric analysis is representative of three Western blot experiments. ^∗P < 0.05, as compared with hypoxia-stimulated nontreated cells.