Skip to main content
. 2009 Nov;175(5):2146–2158. doi: 10.2353/ajpath.2009.090218

Figure 3.

Figure 3

Hypoxia up-regulates Cr-1 mRNA and protein expression through HIF-1α. Real-time PCR for HIF-1α (A) and Cr-1 (B) in mES cells transfected with HIF-1α or control siRNAs. mRNA expression was normalized to 28S expression. Data are representative of three experiments with duplicate samples. ∗P < 0.05, as compared with hypoxia-stimulated nontransfected cells. C: Western blot analysis for Cr-1 and β-actin in siRNA transfected mES cells. D: Fold difference in Cr-1 protein expression by desitometric analysis of Cr-1 in mES cell lysates normalized to β-actin content. Densitometric analysis is representative of three Western blot experiments. ∗P < 0.05, as compared with hypoxia-stimulated nontransfected cells.