Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Exp Dermatol. 2009 Mar 6;18(7):586–595. doi: 10.1111/j.1600-0625.2008.00830.x

Figure 5. UVA irradiation upregulates expression of Gpnmb.

Figure 5

B16F10 cells were irradiated with different doses of UVA (A) or UVB (B and C) and cultured for 24 h (A and B) or 48 h (C). B16F10 cells were also cultured with β-MSH (0.2 µM) plus IBMX (0.1mM) (D) for the indicated time or cultured with varying doses of α-MSH for indicated time (E). Whole cell extracts (20 µg/lane) were prepared from treated cells and assayed by immunoblotting for expression of Gpnmb or β-actin. Expression levels shown under the immunoblotting are expressed as fold increase over control that is calculated by dividing experimental relative expression level (intensity of Gpnmb band/that of β-actin) by control expression level (untreated cells). Data shown are representative of three experiments (A and B) and 2 experiments (C through E).