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Human melanocytes were cultured in the presence of different agents. After culturing for 1 day, cells were stained with 3D5 mAb (open histograms) or control IgG (closed histograms). Staining was examined by flow-cytometry. Mean fluorescence intensity (MFI) of staining by 3D5 mAb is shown in the histograms. Second experiment showed similar results.
