TABLE 1.
Quantitative Statistical Analysis of c-fos Expression in Definite Brain Areas during the Hibernation Bout
| Phase of Hibernation Bout | ART | ET | LT | EA | LA | IBA |
|---|---|---|---|---|---|---|
| Temperature (average, n = 4) | 36.4°C | 23.1°C | 5.4°C | 10.9°C | 32.1°C | 37.3°C |
| Brain regions | ||||||
| Medial preoptic area (MPA) | 78 ± 9 | 95 ± 19 P > 0.05 |
72 ± 12 P > 0.05 |
68 ± 9 P > 0.05 |
149 ± 12 P < 0.001 |
164 ± 17 P < 0.001 |
| Ventromedial subdivision of MPA | 88 ± 9 | 54 ± 7 P < 0.01 |
50 ± 8 P < 0.001 |
111 ± 14 P > 0.05 |
167 ± 12 P < 0.001 |
175 ± 19 P < 0.001 |
| Ventrolateral subdivision of MPA | 59 ± 8 | 121 ± 12 P < 0.001 |
43 ± 6 P > 0.05 |
48 ± 10 P > 0.05 |
59 ± 8 P > 0.05 |
121 ± 20 P < 0.001 |
| Choroid plexus of lateral ventricle | 51 ± 5 | 111 ± 13 P < 0.001 |
239 ± 16 P < 0.001 |
232 ± 14 P < 0.001 |
75 ± 14 P < 0.05 |
41 ± 7 P > 0.05 |
| Ependyma of lateral ventricle | 30 ± 8 | 51 ± 9 P > 0.05 |
139 ± 18 P < 0.001 |
176 ± 17 P < 0.001 |
200 ± 21 P < 0.001 |
31 ± 7 P > 0.05 |
| Suprachiasmatic nucleus | 61 ± 6 | 125 ± 16 P < 0.001 |
195 ± 19 P < 0.001 |
220 ± 20 P < 0.001 |
174 ± 19 P < 0.001 |
144 ± 17 P < 0.001 |
| Reticular thalamic nucleus | 29 ± 6 | 158 ± 10 P < 0.001 |
137 ± 19 P < 0.001 |
190 ± 16 P < 0.001 |
175 ± 13 P < 0.001 |
82 ± 17 P < 0.001 |
| Somatomotor cortex | 128 ± 9 | 54 ± 8 P < 0.001 |
31 ± 11 P < 0.001 |
53 ± 12 P < 0.001 |
60 ± 10 P < 0.001 |
120 ± 12 P > 0.05 |
| Paraventicular nucleus | 65 ± 5 | 81 ± 13 P > 0.05 |
88 ± 14 P < 0.05 |
97 ± 21 P < 0.05 |
141 ± 15 P < 0.001 |
212 ± 25 P < 0.001 |
| Arcuate nucleus | 54 ± 4 | 32 ± 6 P < 0.01 |
34 ± 3 P < 0.01 |
41 ± 8 P > 0.05 |
58 ± 6 P > 0.05 |
145 ± 11 P < 0.001 |
| Dorsolateral hypothalamic area | 71 ± 9 | 56 ± 8 P > 0.05 |
43 ± 9 P < 0.05 |
49 ± 14 P > 0.05 |
69 ± 6 P > 0.05 |
171 ± 19 P < 0.001 |
| Ependyma of 3rd ventricle | 47 ± 9 | 70 ± 14 P > 0.05 |
191 ± 19 P < 0.001 |
230 ± 13 P < 0.001 |
196 ± 18 P < 0.001 |
46 ± 8 P > 0.05 |
| Tanycytes | 45 ± 4 | 169 ± 14 P < 0.001 |
242 ± 26 P < 0.001 |
247 ± 19 P < 0.001 |
169 ± 25 P < 0.001 |
42 ± 9 P > 0.05 |
| Hippocampus | 49 ± 7 | 31 ± 3 P > 0.05 |
44 ± 8 P > 0.05 |
40 ± 9 P > 0.05 |
38 ± 10 P > 0.05 |
43 ± 8 P > 0.05 |
| Choroid plexus of 4th ventricle | 47 ± 8 | 71 ± 13 P < 0.05 |
248 ± 24 P < 0.001 |
205 ± 29 P < 0.001 |
156 ± 23 P < 0.001 |
93 ± 13 P < 0.01 |
| Ependyma of 4th ventricle | 34 ± 3 | 48 ± 9 P > 0.05 |
69 ± 13 P < 0.01 |
160 ± 14 P < 0.001 |
235 ± 19 P < 0.001 |
42 ± 12 P > 0.05 |
| Raphe nuclei | 53 ± 4 | 46 ± 7 P > 0.05 |
41 ± 9 P > 0.05 |
48 ± 11 P > 0.05 |
53 ± 6 P > 0.05 |
61 ± 13 P > 0.05 |
Values are quantitative density data generated by ImageJ software, calculated by identical areas of four different animals and expressed as mean with standard error of mean. Statistical significance among the different phases of hibernation bout is calculated by one-way ANOVA with Bonferroni post-hoc tests and P-values expressed for every studied brain region in comparison with measurement values in the ART group.