TABLE 3.
Results of H4 acetylation (score) in tumors and spleens from vehicle control mice or belinostat-treated mice with A2780 xenografts (Experiment 4)
| A2780 | H4 acetylation |
|||||
|---|---|---|---|---|---|---|
| Belinostat, 200 mg/kg i.v. |
||||||
| ID | Vehicle, 15 min | 15 min | 30 min | 1 h | 2 h | 3 h |
| Tumor | ||||||
| 270 | No (0) | |||||
| 478 | No (0) | |||||
| 282 | No (0) | |||||
| 524 | Weak (1) | |||||
| 277 | Moderate (2) | |||||
| 531 | Moderate (2) | |||||
| 296 | No (0) | |||||
| 273 | Moderate (2) | |||||
| 286 | Moderate (2) | |||||
| 523 | Strong (3) | |||||
| 527 | Weak (1) | |||||
| 511 | Strong (3) | |||||
| 283 | Strong (3) | |||||
| 525 | Strong (3) | |||||
| 508 | Strong (3) | |||||
| 287 | Moderate (2) | |||||
| 285 | Strong (3) | |||||
| 522 | Moderate (2) | |||||
| 532 | Moderate (2) | |||||
| 295 | Weak (1) | |||||
| 279 | Moderate (2) | |||||
| Mean | 0 | 1.25 | 2 | 3 | 2.33 | 1.67 |
| Spleen | ||||||
| 270 | No (0) | |||||
| 478 | No (0) | |||||
| 282 | No (0) | |||||
| 524 | Moderate (2) | |||||
| 277 | Moderate (2) | |||||
| 531 | Weak (1) | |||||
| 296 | Weak (1) | |||||
| 273 | Strong (3) | |||||
| 286 | Strong (3) | |||||
| 523 | Moderate (2) | |||||
| 527 | Moderate (2) | |||||
| 511 | Strong (3) | |||||
| 283 | Strong (3) | |||||
| 525 | Moderate (2) | |||||
| 508 | Strong (3) | |||||
| 287 | Moderate (2) | |||||
| 285 | Moderate (2) | |||||
| 522 | Moderate (2) | |||||
| 532 | Weak (1) | |||||
| 295 | No (0) | |||||
| 279 | Weak (1) | |||||
| Mean | 0 | 1.5 | 2.5 | 2.75 | 2 | 0.67 |
No H4 acetylation is observed in tissue from vehicle control mice. Weak-to-moderate acetylation is observed after 15 min with maximal levels after 1 h. The levels of acetylation decrease after 2 h.