Fig. 1.

Illustration of four different modes of dendritic cell (DC) loading and immunization. (A) All seven peptides were loaded onto the DCs simultaneously and injected intracutaneously (i.c.) in one flank of the mouse. (B) The same number of DCs was aliquoted in seven vials and pulsed separately with one peptide for each vial and mixed in one syringe followed by i.c. injection in one flank of the mouse. (C) Peptides were loaded separately onto DCs as described in group B, but prior to injection, epitopes ranked 1–3 (Table 1) were pooled and administered in the left flank of the mouse and epitopes ranked 4–7 (Table 1) were pooled and administered in the right flank of the mouse. (D) Epitopes ranked 1–3 and 4–7 (Table 1) were loaded onto DCs in two separate vials followed by i.c. injection in the left and right flanks of the mouse, respectively.