Fig. 2.

Two of seven epitopes dominate the T-cell responses. Chromium release cytotoxic T lymphocyte (CTL) assay and IFN-γ ELISpot assay were used to measure the breadth and magnitude of T-cell responses. (A) (B) (C) and (D) are illustrated in Fig. 1A–D, respectively, and explained in the legend to Fig. 1. Groups of six mice were used for immunization and pooled splenocytes were tested against all seven CTL epitopes and one irrelevant negative control peptide (mock). SFU, spot-forming unit. One representative experiment of three is shown.