Table 1.
Immunogenicity of seven HIV-1-derived HLA-A*0201-binding synthetic peptides
| Epitope | Sequence | Rank1 | IC50 (nM) | Specific lysis (%)2 | SFU/mio splenocytes3 | IFN-γ+TNF-α+4 |
|---|---|---|---|---|---|---|
| Gag150mod | RLLNAWVKV | 1 | 26 | 66 ± 1.2 | 19657 ± 1774 | 16.6 |
| Gag433 | FLGKIWPS | 2 | 3 | 49 ± 2.7 | 5714 ± 575 | 12.6 |
| Vif23(9V) | SLVKHHMYV | 3 | 168 | 46 ± 3.6 | 4217 ± 146 | 5.0 |
| Pol606mod | KLGKAGYVV | 4 | 384 | 36 ± 3.7 | 4391 ± 370 | 3.2 |
| Env67(2I) | NIWATHACV | 5 | 103 | 28 ± 0.8 | 3658 ± 223 | 3.6 |
| Vif101(9L) | GLADQLIHL | 6 | 219 | 17 ± 0.9 | nd | 2.5 |
| Vpu66mod | ALVEMGHHV | 7 | 334 | No lysis | nd | 2.2 |
Values represent data from groups of four mice with pooled splenocytes. HIV-1; human immunodeficiency virus type 1; HLA, human leukocyte antigen; SFU, spot-forming unit; IFN-γ, γ-interferon; TNF-α, tumor necrosis factor-α; nd, not determined.
1
Epitopes are ranked according to decreasing immunogenicity.
2
Mean values at effector target ratio 50:1, ± indicate SD of triplicates.
3
Mean values, ± indicate SD of triplicates.
4
Values represent percentage double-positive IFN-γ and TNF-α-producing CD3+CD8+ T cells from pooled splenocytes (n = 4 mice) in order to obtain sufficient cell counts for optimal analysis.