Skip to main content
. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Circulation. 2008 Oct 27;118(20):2063–2072. doi: 10.1161/CIRCULATIONAHA.108.788711

Figure 1.

Figure 1

Figure 1

Figure 1

Transfer of T-cells into WT mice. Mice were immunized with mcTnI or adjuvant alone. On day 21 T-cells were isolated from splenocytes and re-stimulated in vitro in the presence of dendritic cells and monocytes with 10μg/ml of mcTnI (mcTnI/mcTnI or no/mcTnI) for 48h or were not re-stimulated with mcTnI (mcTnI/no or CFA/no). Then 106-107 T-cells per mouse were transferred to WT mice irradiated with 600 rad. Effects on production of mcTnI specific autoantibodies (total IgG (a), mean ± SEM) and cytokines ((b), mean ± SEM), on inflammation and fibrosis (c), on left ventricular end-diastolic ((d), mean ± SEM) and end-systolic diameter ((e), mean ± SEM) on fractional shortening ((f), mean ± SEM), and on expression of cytokines and cytokine receptors in the myocardium (g). Antibody endpoint titers for each individual mouse were calculated as the greatest positive dilution of antibody above normal mouse serum levels for day 21. *: p<0,05 (Kruskal-Wallis test and U test).