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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Am J Transplant. 2009 Jul 28;9(10):2292–2302. doi: 10.1111/j.1600-6143.2009.02781.x

Figure 6. IFN-γ participates in the indirect activation of STAT1 by LMP1.

Figure 6

A. B95.8#1 and B95.8#4 BL41 clones were stained with anti IFNR1-PE, IFNRII-PE (filled-in histogram) or isotype controls (open histogram) and analyzed by flow cytometry. B, B95.8#1 and B95.8#4 cells were crosslinked for 0 or 24 hours with/without pre-incubation with either 2 μg/ml of anti-IFNRI and 20 μg/ml of anti-IFN-γ, 22 μg/ml mouse IgG or 1 μM Bay 11-7082. Cells were harvested and lysed in phospholysis buffer and immunoprecipitated for STAT1. Western blotting with anti-p-TyrSTAT1 and anti-STAT1 antibodies was performed. Densitometry is shown below the blot and represents the ratio of p-Tyr-STAT1 to total STAT1. Data is representatitive of three independent experiments.